A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus)

TitleA Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus)
Publication TypeJournal Article
Year of Publication1998
AuthorsKocher, TD
Secondary AuthorsLee, W-J
Tertiary AuthorsSobolewska, H
Subsidiary AuthorsPenman, D, McAndrew, B
Start Page1225
Date Published1998

We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of ~ 1000–1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and

quantitative traits in cichlid fishes.