A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus)
Title | A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus) |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | Kocher, TD |
Secondary Authors | Lee, W-J |
Tertiary Authors | Sobolewska, H |
Subsidiary Authors | Penman, D, McAndrew, B |
Journal | Genetics |
Volume | 148 |
Issue | 3 |
Start Page | 1225 |
Pagination | 1225-1232 |
Date Published | 1998 |
Abstract | We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of ~ 1000–1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes. |
URL | http://www.genetics.org/content/genetics/148/3/1225.full.pdf |